![]() coli but did not modify PTE’s enzymatic activities 24. These mutations improved the levels of soluble and functional enzyme in E. PTE-S5 carried three mutations (Lys185Arg, Asp208Gly and Arg319Ser) compared with the wild-type PTE. A PTE variant with higher expression in Escherichia coli, dubbed PTE-S5, was previously evolved via random mutagenesis and screening with 2-naphthyl acetate 24. The early steps of PTE’s evolution into an arylesterase have been described previously 24, 25, 26. By producing and characterizing this evolutionary trajectory, we provide a quantitative description of the magnitude of diminishing returns and tradeoffs throughout this trajectory, and also describe the molecular basis of diminishing returns and tradeoffs. We isolated and characterized the intermediates of this process, and could thus describe the entire trajectory. Iterations of random mutagenesis and selection for variants exhibiting higher arylesterase rates led to the gradual fixation of 18 substitutions, and a highly efficient arylesterase ( k cat/ K M=1.7 × 10 7 M −1 s −1). The laboratory evolution began from the weak, promiscuous, arylesterase activity of wild-type PTE ( k cat/ K M with 2NH=4.2 × 10 2 M −1 s −1). Although the original (PTE) and new (arylesterase) enzymes catalyse hydrolysis, the reactions differ in the bond that is hydrolysed (P–O versus C–O), the shape and binding orientation of the substrates, and the geometry of the substrates (tetrahedral versus planar) and transition states (TSs) (pentavalent versus tetrahedral Fig. Here, we describe a complete evolutionary trajectory comprising a functional transition from a naturally occurring phosphotriesterase (PTE) from Pseudomonas diminuta that catalyses the hydrolysis of the pesticide paraoxon with high efficiency ( k cat/ K M=2.2 × 10 7 M −1 s −1) 22, 23, to an arylesterase that catalyses the hydrolysis of 2-naphthyl hexanoate (2NH Fig. Although these experiments provided novel insight, they involved both random and rational (targeted) mutagenesis and/or varying substrates along the trajectory 21. Marked switches in enzymatic functions have been achieved via laboratory evolution, whereby the catalytic efficiency of the newly evolved enzyme matched that of the starting point 20. Such experiments have shown how new functional traits evolve, that is, by mutations increasing a latent, weak, promiscuous activity 11, 16, 17, 18, 19. Laboratory evolution (also dubbed experimental or directed evolution) can allow us to characterize every intermediate in a trajectory and thereby help us to understand the mechanisms and driving forces of evolution 13, 14, 15. In addition, there is no way to reproduce the series of evolutionary intermediates that underlie the divergence of natural enzymes. The starting points of natural enzymes remain mostly unknown. ![]() Overall, data and insights regarding complete functional transitions in enzymes are scarce. Finally, the potential implications of diminishing returns and tradeoffs on the existing repertoire of biomolecules, and of enzymes in particular, also remain unidentified. Our understanding of the molecular basis for diminishing returns and tradeoffs is also limited. However, exactly how diminishing returns and tradeoffs affect a complete evolutionary trajectory involving the emergence of a new catalytic activity remains unknown. Similarly, functional tradeoffs have been studied in the context of protein evolution 3, 10, 11, 12, yet previous works have focused on the early steps of optimization. For instance, it has been shown that the first mutations have the largest impact, and diminishing returns have been observed with respect to metabolic pathways 5, bacterial evolution 6, population sizes 7, mutation rates 8 and the evolution of antibiotic resistance 9. These aspects of evolutionary optimization have been addressed 3, 4, yet our understanding of the processes that define evolutionary optimization is by no means complete. Tradeoffs: the improvement of the trait under optimization comes at the expense of other traits. ![]() Diminishing returns: the first steps yield larger improvements than the later steps 1, 2, meaning that the closer the process is to its optimum, the slower and less cost-effective optimization becomes. Graduality: each step provides an incremental improvement. Optimization processes, be they industrial, economical, computational or biological (evolutionary), share several common features.
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![]() It’s simply beyond Mayer’s power because no matter how much money she has, Google and Facebook will always have more. That’s because that trajectory is determined more by Google and Facebook and by changes in the ad market than by anything Yahoo can do. Lots of acqui-hires (buying tech companies for their people) and big acquisitions like Tumblr have not significantly changed the company’s downward trajectory. Mayer seems to be trying to buy her way ahead of the next technology wave, but having been at this game for a couple of years so far, it isn’t going well. The only other possible course for Yahoo, in my view, is to turn the company into a Silicon Valley version of Berkshire Hathaway. One is to wind things down and return Yahoo’s value to shareholders in the most efficient fashion, selling divisions, buying back shares, and issuing dividends until finally turning out the lights and going home. In practical terms there are only two logical courses of action for Mayer and Yahoo. Even if she fires everyone, Yahoo still has a funny smell. She has the temperament for it but the rest of Yahoo does not. It’s not that Mayer isn’t super-smart, it’s that the job she is attempting to do may be impossible. There is no reason to believe that more money is the answer. Yahoo would have to do what it has been trying to do ever since Tim Koogle left as CEO in 2003 and regain its mojo. Those ships have, for the most part, already sailed and can never be caught. Yahoo CEO Marissa Mayer, thinking like Type A CEOs nearly always seem to think, wants to take some of the billions reaped from the Alibaba IPO and dramatically remake her company to compete again with Google, Microsoft, Facebook, and even Apple. ![]() But - if it will - Yahoo could invest its way to even greater success. Having covered Yahoo continuously since its founding 20 years ago it is clear Y! has little chance of managing its way out of this latest of many crises despite all the associated cash. Yahoo’s Wall Street honeymoon, if there ever was one, is over, leaving the company trying almost anything it can to avoid sliding into oblivion. Alibaba’s IPO has come and gone and with it Yahoo has lost the role of Alibaba proxy and its shares have begun to slide. ![]() It will provide you with an average ping of 100ms and in areas, with advanced infrastructures, it can get close to 0ms. It is better to get a good internet connection to experience smooth gaming – at least 10 MBS internet speed is required. The ping is crucial to win games in any e-sport title, but in CS:GO it has by far the strongest impact! Low ping is necessary to play and react good in Counterstrike, the gaming devices also often need translation time (like the monitor who often needs 2ms – 20ms to print the actions onto your screen).īad Ping with serious problems and delay! Ping is the time between the send out of information from the source to the connector: If you play CS:GO the timing between these two factors describes how fast the actions are translated on your end device, while zero (0ms) would be 100% live. Servers are mostly stable in general, and in the first place, a high ping originates from the user side. Ping is based on the internet connection of the user to the server. The lower ping you have, the smoother your game will run. Ping is the latency that you have while playing, which is dependent on the quality of the internet connection you are using to play CS:GO. What does Ping means and which Ping is normal in CS:GO? Now you can check your CS:GO ping and always keep an eye on it.Now enter “net_graph 1” to show the ping: Join a CS:GO game and open the console (in my case with the key F5).Set the action to a key, I set the key to F5, whenever I click on F5 the console will open and close: Now click on Keyboard/Mouse settings and scroll down to “Toggle Console”.Click on it and set it to “yes” to use the Console. In the settings choose the “game” tab and scroll down to “ Enable Developer Console“. To activate the Console in CS:GO, launch the game and open the game settings by clicking onto the wheel on the left side. First of all we need to activate the CS:GO Console, if you have already done that you can go on to step 3.On this image I have a ping of 15ms: Option 2 to see the Ping in CS:GO: ![]() To see the ping just hold the TAB-button and view the ping number on the left side which is shown in ms. It’s super easy to see the ping in CS:GO, here is a quick tutorial, just follow the simple steps to show the ping ingame: Option 1 to see the Ping in CS:GO:īy default, you can see the ping of all players in an active game in the match statistics. How to see your Ping in Counterstrike CS:GO? While older versions of Whatsapp let you manually enter the verification code received on another device, WhatsApp no longer lets you enter the verification code manually. Once that is done, you can install most games and apps - some, which rely on a working SIM card for confirmation, such as WhatsApp, won't run on your PC. 5) You'll have to log in using your Google account once you search Google Play for apps. ![]() Click the big search icon at the top > type the name of the app > select "Search Play for." to get the apps from Google Play.
![]() How to create an Android project in Eclipse and run it If we use Libgdx to develop in Android, we will have an HTML5 version with almost no extra work. GWT is a tool for developing in Java which is compiled into HTML5. In the future I will convince you to use them.Īpp Engine is a free server for Java which is very convenient for Android or Web applications. With this, we have all we need for Android development but I suggest downloading "Google Plugin for Eclipse" and the SDKs for App Engine and GWT. We select all options in "Development Tools" except "Native Development Tools". Add ADT "" using " Google Plugin for Eclipse". After indicating in which directory we want to set the workspace, go to the Help menu - Install new Software. We download Eclipse Kepler, unzip it in the folder you want and start eclipse.exe. Chose "Kepler" as there are conflicts between Eclipse later versions and plugins used for developing Android and Java in general like Gradle, Libgdx and others. We look for "eclipse download" in google and navigate to find "Eclipse IDE for Java Developers" but instead of the latest version, look for the one called "Kepler". Installing Eclipse and Android ADT plugin (any doubt go to Windows and MS-DOS Environment variables) Gradle) to define the ANDROID_HOME variable with the directory where you installed the SDK. ![]() We run the SDK manager and download the packages selected by default. We download the SDK and install it in our PC. In this tutorial we will install stand-alone SDK, Eclipse Kepler and finally the Eclipse plugin. (This is the preferred choice of Android developers in general but not for Google which has the option somewhat hidden). Stand-alone SDK Tools: Consisting only of the Android SDK.Android Studio: it is an adapted version of IntelliJ for Android.(The advantage is that the installation is faster). Eclipse ADT: A package of the Eclipse Juno version with the ADT plugin installed, which comes with an Android SDK.At the time I am writing this tutorial, options are: On the Android website there are several options for downloading the Android SDK development tools. You can order all kinds of stamps from VistaPrint, including self-inking stamps, pre-inked stamps, notary stamps, signature stamps, date stamps and replacement ink. I’ve been using VistaPrint for self-inking stamps for years, and you can get them for less than $20. These kinds of stamps ordered through a bank can cost between $40-$50. And if not, you have to do it manually with a stamp. Some check scanners do this automatically as you scan them in. A typical stamp reads, “Pay to the order of for Deposit Only.” It usually includes your company name and account number as well. If the Primary Attorney In Fact is deceased, please provide a copy of the death. If you remotely deposit checks for your business, you’re probably required to stamp them first. If physician statement(s) are required, Boeing will require a copy. They were about half the cost of the checks I’d bought directly through previous banks! After using them for a year, I can tell you that they’re printed on good paper, the 2-part duplicate feature works great, and I will definitely reorder when the time comes. Replacement Plastic Divider for Duplicate Checks (Pack of 2) 4.3 out of 5 stars 3. I was also able to add the Blue Gurus logo to the design. I ordered general purpose, 2-part business checks (in blue of course) and a binder to keep them organized. So you will end up going to if you decide to order checks.) ![]() (A third party called Harland Clarke Check Printing actually handles the ordering and printing of the checks. To submit a claim, you will need a copy of the itemized receipts or service statements for each patient that includes. I’m a big fan of Costco… so I was definitely willing to give them a shot. ![]() ( Check out her LinkedIn profile… I love the summary she wrote starting with “ You can take the Girl out of the small town, but you can’t take the small town out of the Girl!”) When I asked Tricia to order me checks, she told me that an excellent place to get business checks is through Costco. There are lots of reasons I like to bank with Core Bank, and one of them is that Tricia Luedke takes good care of me. In the previous two banking relationships, I ordered checks directly from the bank. Pricing will vary depending on size, quantity, and finishing upgrades. I made that change about a year ago, and needed to get business checks for the new account. Price shown is the starting at price for our whole assortment of Custom Checks. They’re a blogging client of mine and I prefer to do business with companies that are doing business with me. The Blue Gurus checking account is at Core Bank. And you know I like to share these kinds of tips, so here we go! Business Checks – Costco But they’re usually necessary to have when running a business. Business Checks and Remote Deposit Stamps aren’t sexy or exciting. Costco Executive Member: 12.90.Product Details Singles Duplicates Quantity: 100 Checks Per. It was hard for me to come up with an enticing title for this story. Quantity: Style/Color: Costco Gold Star/Business Member: 16.12. Of course, most shoppers don't tend to take issue with this practice in the first place, but receipt scanning software could serve as a valuable resource for those who wish to make stringent records of their spending and savings.Business Checks and Remote Deposit Stamps? Costco and VistaPrint! In fact, the video concludes with the user explaining that they have found a method for digitally scanning their receipt on their cell phone, circumnavigating the need to save the physical receipts once they leave the store. One video comment reads, "You could ask the cashier for a duplicate," while another states, "I could see if like we were forced to shop at Costco but they're Costco rules if you don't follow them don't shop at Costco."Īdditionally, the increase in electronic purchases and advancements in technology have contributed to doing away with the old Sharpie method that the original poster loathes so much. #LossPrevention #Costco #Receipt #OCR #Scanner #infosec #cybersecurity #socialengineering #kerneldump original sound – KernelDump Will have to pry my sales receipt out from my cold unalived hands. ![]() to 2:00 p.m., and Saturday and Sunday brunch from 11:00 a.m. Meril is currently open for dinner nightly starting at 5 p.m., lunch Friday from 11:30 a.m. Please note that The Attic is not ADA accessible and multiple stairs lead up to the space. Reservations are required and can be made by calling (504) 526-3745 or on OpenTable. From there, you’ll be seated in The Attic for brunch.Įvent is nonrefundable, nontransferable, and guests must be 21+ to attend. Featuring some of your fabulous favorites from the epic Ru Pauls Drag Race, Drag Diva Brunch adds a bit of fun and sparkle to your weekly brunch. On the day of the event, please visit the host stand to check in with a photo ID (or copy of the photo ID) that matches the name of the person who purchased the tickets, as well as the confirmation email associated with the order. The Drag House Party Brunch House of Blues New Orleans The Drag House Party Brunch READY TO SEE A GREAT SHOW Find Tickets Now Upgrade to VIP Restaurant and Bar Dining Reservation Foundation Room Guest List Foundation Room VIP Dining Event Time: 11:00 AM Prices: 55.00 to 60. Please note that tables seat 8 guests – should you have less than 8 in your group other parties may also be seated at your table. We strongly suggest purchasing tickets on a single order for groups who wish to sit together. Limited seating is available and advanced purchased tickets are required for entry. The Attic opens at 10am with brunch starting at 10:30am and the show beginning at 11am sharp. There’s no extra charge for the show, but plan ahead, because reservations book up months yes, months in advance. Much like New Orleans, we are here to tempt you with a mlange of flavors from food, wine, and spirits to live music on weekends. Drag Brunch features an a-la-carte menu, bottomless mimosas, and an impressive Bloody Mary bar. The Tasting Room New Orleans is a wine and cocktail bar with an eclectic menu located in the Lower Garden District. ![]() And of course, entertainer tips are customary and appreciated - please tip your divas!īrunch buffet includes: fried chicken and waffles, shrimp and grits, scrambled eggs, bacon, sausage, biscuits, and home fries.Īdd on bottomless mimosas for $10/person.Īdd on bottomless bloody marys for $10/person. This long-running spectacle is held every Saturday and Sunday with seatings at 10 a.m. Jessica Emms! Please note that alcohol is not included in the cost of your ticket and will be sold separately. ![]() Emms & Her Fabulous Femmes will put on a show for the ages! Tickets are $45 (tax and tip included) and includes a brunch buffet as well as a front row seat to the divas – Giselle Trivianni, Vanessa Carr Kennedy, Rue B. Bianca Del Rio is a dimple-cheeked, larger-than-life drag queen and. Brace yourself for the finest drag show around accompanied by an amazing, and surprisingly affordable, brunch menu. Join us in The Attic at Lucy’s – New Orleans for the best Drag Brunch in town! She is a fixture in the New Orleans and New York City club scenes, often working. Classic Creole Cuisine In The Heart of the French Quarter Open for Dinner Tues - Sat 5:30pm to 9:00pm Sunday Jazz Brunch 10:00am to 1:30pm Newsletter Signup. ![]() Now you know how to stay more secure and private online (for free!), but what about device performance? Avira offers numerous cleanup and optimization tools to help you speed up your machine and free up more storage space. ![]() ![]() VPNs create a virtual private ‘tunnel’ through which all your online data is passed, where it’s safer from prying eyes and cyber criminals. Avira Safe Shopping offers a price comparison tool so you can still grab a bargain and rest assured it’s from a trusted site.Īvira Phantom VPN encrypts your data traffic, helps anonymize your online activities and lets you surf more securely, even on public Wi-Fi. Google user and online shopping fan? We have something for you too. It also helps stop potentially unwanted applications (PUAs) from hiding in your downloads-once inside your system, they could spy on you or display an annoying stream of ads. Plus, its anti-tracking capabilities can help prevent ad networks from monitoring your online activities. It highlights infected sites in your search results, so you’re forewarned. If you love surfing online, consider this free, discreet add-on that helps secure your browsing and can protect your privacy: Avira Browser Safety (for Opera, Firefox, and Microsoft Edge) was designed to shield you from dangerous and phishing websites, malicious advertisements, and online tracking. Our Avira Free Antivirus is a great place to start if it’s just free software security you’re after: It offers cloud-based, real-time protection to help block a huge range of online threats and malware, including the latest viruses, Trojans, and ransomware. That's why we provide free software for your online security and privacy, plus performance enhancement-for Windows, Mac, Android, and iOs devices. At Avira, we believe that everyone should enjoy a safer and more private digital life-anytime, anywhere. A recent industry report on landsat satellite imagery data estimates that total annual value to the economy of 2.19 billion, far exceeding the multi-year total cost of building, launching, and managing Landsat satellites and sensors. Results for this API are returned data in JSON format, so we passed the data through a JSON parse tool, then further parsed the data using a text to columns tool.įinally, we filtered out the metadata and we had our complete data set. Adds picked object registered with this PickSupport that are drawn at the specified pick point or intersect the draw contexts pick rectangle to the draw contexts list of picked objects. Landsat imagery is provided to the public as a joint project between NASA and USGS. Used a text input tool to paste in the URL, along with our user-specific API key and other parameters (such as start and end date) required to make the request.Īfter the full URL is passed to the download tool, http action is set from the payload tab. We then:ĭetermined the URL needed and the http protocol for making the data request. We decided to protect our fellow citizens from possible doom due to an asteroid or other space object by analyzing data from the Near Earth Object (NEO) Feed with Alteryx.Īfter applying for API access on NASA’s website we searched for the specific documentation for the NEO data feed. By and large, the shapes of features on Earths surface are represented in Planet APIs by GeoJSON geometry objects. Appreciate if anyone help shed some light on how to parse the GeoJSON response in node JavaScript. The issue is when the response data is GeoJSON. filename Datasets/eqdata30daym1.json with open (filename. When I replace the URL with something that returns JSON it works. Code: import json from aphobjs import Scattergeo, Layout from plotly import offline Explore the structure of the data. Im at the beginner stage so please suggest me as simple as possible. Pressure > Atmospheric Pressure Measurements Earth Science > Atmosphere > Atmospheric Pressure. I have no idea whats wrong with the code, its showing blank on the browser. NASA’s Open Innovation team works to make the space agency’s data freely available to the public. Convert a CF Standard Name to/from a GCMD Science Keyword. To illustrate how easy it is to use an API key to integrate external data with Alteryx, let’s look at a fun exercise we recently completed using a publicly-available data feed from NASA. Whether it’s an ongoing feed from Bloomberg or a one-time query of survey data, the use of external data sources can improve your decision-making abilities.ĭevelopers often integrate disparate technologies using an Application Programming Interface (API). At Continuus, most of our clients combine internal data with information from outside sources. GeoJSON is a subset of the JSON format and can be parsed natively by JavaScript and in most modern software. Mapbox web services and APIs serve geospatial data as GeoJSON. These insights can lead to faster and smarter decisions. GeoJSON is an open standard file format for representing map data. ![]() Self-serve data science helps businesses use their own data to ask and answer questions. ![]() It must have been about that time that all of Microsoft's developers got SSDs on their desk (I'm only half joking). Vista leaned a lot more heavily on disk I/O that its predecessors, so turning off Windows Search, and a few other things I forget made it tolerable to use. The machines sold as Vista-capable weren't all that great with XP, but better, so were even under-powered for the previous generation. So much so, that I could use a Vista 64-bit machine as an alternative to Windows 7 if it were still supported. I used two sets of Vista machine, the 32-bit machines were almost all terribly slow, the 64-bit machines were surprisingly capable. Here are the names of the 75 programmers that have worked on ReactOS: My hat is off to the ReactOS developers, and congratulations on the latest release. This is one of the most important software projects in history. So many untold man-hours of thankless work going into the project, and the vast majority of that "hard" work with no payoff for years.Īnd now it's finally getting close to actual Windows, after decades of work, and soon (well, years, but still) people will be using it everywhere as a replacement for Windows. Meanwhile, work keeps going on in the background. And the second-hand stories I've heard about people who did install React was basically "I was bored, I put it on a drive, played around for 5 minutes, and wiped it."īut that doesn't discourage them. Hell, I have never seen a ReactOS installation in the wild, and I'm the kind of person whose friends install Haiku, Nix and NetBSD as their daily drivers. There were people working on this two decades ago, putting in tiny fixes and little bits of code and whatnot, and they did it even knowing that basically nobody would see their work for decades. The 0.4.0 release aims to improve compatibility with USB devices, simplify connecting to WiFi networks and improve sound support a final release date is not available.This has been one of my favorite software projects to watch, because it's such an insanely monumental undertaking and moves so damn slowly. ![]() As older apps break on newer versions of Windows, for example, ReactOS could become the go-to system for running them. Even if they never achieve full compatability with all current Windows app, which is a fast-moving target, ReactOS could wind up being useful in ways that are hard to predict. It might seem quixotic, but the ReactOS team keep pushing themselves to build a free version of Windows almost from scratch. Users have tested it, uploading videos of programs they’ve managed to make work (and some they haven’t). The project’s homepage claims it’s only “recommended only for evaluation and testing purposes. Diablo II, for example, is a game released in 2000 that apparently “installs fine” but “doesn’t run past character selection.” Videos show web-based software, like GOG’s online store, fail to work to varying degrees.ĮactOS isn’t ready to be your primary operating system. So are a few prominent Windows-only programs, ike WinRAR.īut that’s not to say everything is working. It notes the Windows versions of many open-source stalwarts, including Firefox and Libre Office, are already up and running. ![]() In theory this could be revolutionary, but in practice rebuilding Windows from scratch isn’t simple.įor evidence of this you need only look at the list of programs tested on ReactOS 0.4. ReactOS has always been unique because of its goal –full compatibility with Windows software. There are plenty of free, open source operating systems on the market, including hundreds of Linux distributions. ReactOS, an operating built from scratch to be binary-compatible with Windows programs and drivers, is nearing its 0.4 release - you can download the release candidate right now. The dream of an open source replacement for Windows is still alive after 17 years of development. |
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